periplasmic space การใช้

• The " Bdellovibrio " cell then enters the host periplasmic space.
• They accumulate in the periplasmic space.
• Each motor element appears to be localized to the periplasmic space and is bound to the peptidoglycan layer.
• BPs are soluble proteins located in the periplasmic space between the inner and outer membranes of gram-negative bacteria.
• Many important electron transport occur across the cell membrane between the cytoplasm and the " periplasmic space ".
• In the process the reducing power of the QH 2 is used to pump protons across the membrane to the periplasmic space.
• The osmotic pressure of the periplasmic space can be simply altered by changing that of the medium as osmolytes freely penetrate the outer membrane.
• In prokaryotes, most of the chemical reactions of metabolism take place in the cytosol, while a few take place in membranes or in the periplasmic space.
• Such an export of the fusion protein into the periplasmic space enables the formation of disulfide bonds in the passenger protein, e . g . antibody fragments.
• Researchers have suggested that AT moves from its site of synthesis to the periplasmic space, where it binds exogenous trehalose to internalize it and hydrolyze it in the vacuoles.
• Inclusion bodies are dense electron-refractile particles of aggregated protein found in both the cytoplasmic and periplasmic spaces of " E . coli " during high-level expression of heterologous protein.
• Unlike " Bdellovibrio ", a genus in the Deltaproteobacteria " Micavibrio " do not invade the periplasmic space of their prey, but feed by attaching themselves to its surface.
• Recently it has been shown that more than 90 % of AT activity in " S . cerevisiae " is extracellular and the hydrolysis of trehalose into glucose takes place at the periplasmic space.
• Exporters and antiporters of the MFS family follow a similar reaction cycle, though exporters bind substrate in the cytoplasm and extrude it to the extracellular or periplasmic space, while antiporters bind substrate in both states to drive each conformational change.
• In E . coli, the TolQRA acts to translocate the Ff phage into the periplasmic space, where a possible membrane fusion event leads to the insertion of the Ff genome into the " E . coli " cytoplasm.
• Proteins which have disulphide bonds are often not able to fold correctly due to the oxidizing environment in the cytoplasm which prevents such bond formation, and a possible solution is to target the protein to the periplasmic space by the use of an N-terminal signal sequence.
• It is characterized as sphere-shaped, has a cell diameter ranging from 0.6 to 1.5 micrometers, a cell envelope consisting of a cytoplasmic membrane, a periplasmic space, and a thin electron-dense layer, and tufts of polar flagella.
• Other bacteria, such as most spirochetes, have two or more specialized flagella ( endoflagella ) arising from opposite poles of the cell, which together constitute the so-called " axial filament " that is located within the periplasmic space between the flexible cell wall and an outer sheath.
• They have a previously unknown cell envelope structure a cytoplasmic membrane, a periplasmic space ( with a variable width of 20 to 400 nm, containing membrane-bound vesicles ), and an outer membrane ( approximately 10 nm wide, resembling the outer membrane of gram-negative bacteria ).
• When the recombinant MBP includes a signal peptide, the fusion protein can be exported into the periplasmic space, which facilitate its purification since the periplasmic fluid contains only a limited number of proteins and can be recovered either by an osmotic shock or by permeabilization of the outer membrane with antibiotics, e . g.